LB Broth (Lennox) Low-Salt 250 g – liquid medium for culturing bacteria

LB Broth (Lennox, Low-Salt) – a medium for sensitive bacterial cultures

LB Broth (Lennox, Low-Salt Version) is a classic, liquid culture medium in the form of a dry powder, intended for the multiplication of bacteria, especially strains Escherichia coli used in molecular biology, microbiology, and biotechnology. The reduced sodium chloride content makes the medium particularly recommended for salt-sensitive experiments and for applications requiring a higher pH compared to classic LB. The product, available in 250g bottles, allows for flexible preparation of the required volumes of medium, optimizing labor costs in both routine and research laboratories. For safe inoculation of cultures, we recommend sterile loops individually packed available from Googlab Scientific.

Composition, preparation and parameters of LB Broth medium

LB Broth (Lennox, Low-Salt Version) is formulated with a balanced composition of 10 g tryptone, 5 g sodium chloride, and 5 g yeast extract per liter of prepared medium, at a final pH of 7,5 ± 0,2 (25°C). To prepare the medium, weigh 20 g of dry powder, add distilled or deionized water to 1 liter, and then heat with stirring until the ingredients are completely dissolved. After pouring into flasks or tubes, the medium is autoclaved at 121°C for 20 minutes, obtaining a clear and stable medium that supports repeated bacterial growth.

Quality control, storage and shelf life

Every batch of LB Broth (Lennox, Low-Salt Version) is quality controlled using reference strains Escherichia coli ATCC 25922, DH5α, and TOP10, incubated at 36 ±1°C for 12–18 hours. The acceptance criterion is the achievement of typical, uniform broth turbidity, confirming the proper culture properties of the medium. The powder should be stored at 2–30°C, in the original, tightly closed packaging, protected from moisture. The shelf life is 36 months from the date of production, which guarantees the stability and reproducibility of the medium parameters throughout its life.