NEST Cell Culture Inserts, Sterile test plates + inserts with PET membrane, for cell culture, TC-Treated

Cell Culture Inserts - PET membrane, TC-Treated

NEST Cell Culture Inserts with membrane PET (polyester) and surface TC-Treated These are professional cell culture systems designed for studying cell transport, migration, invasion, and polarization. The inserts are integrated with 6-well, 12-well, and 24-well plates, and are also available in a 100 mm vessel configuration, making it easy to match the format to the scale and type of experiment. For daily work, including media preparation and chamber maintenance, we recommend accessories from the following sections: pipetting and dosing, including NEST serological pipettes, which facilitate sterile and rapid transfer of media.

PET membrane parameters – pore diameters, microscopic observation and repeatability

The PET membrane is distinguished by its excellent transparency and high optical clarity, facilitating direct observation of cell morphology and condition under brightfield and fluorescence microscopy. Additionally, it has low protein binding, which reduces analyte loss and facilitates reproducible results in transport and cytotoxicity assays. Various membrane pore diameters are available – 0,4 µm, 1,0 µm, 3,0 µm and 8,0 µm – which enables precise model matching to the application: from barrier models (e.g., Caco-2, MDCK) to tests of leukocyte migration, endothelial cells, or cancer cell invasion. To increase work safety and minimize the risk of contamination, it is recommended to use pipette tips with filter and preparation of solutions using PES syringe filters 0,22 µm.

Quality, sterility and ergonomics – Tyvek® packaging, E-Beam and automation compatibility

Surface TC-Treated is modified by vacuum plasma discharge, which gives it a hydrophilic character and a negative surface charge, supporting the adhesion, spreading, and proliferation of most cell lines and ensuring stable culture conditions. The PET membrane inserts are individually packaged in Tyvek® blisters with a peel-off lid, sterilized by E-Beam (SAL 10⁻⁶) and manufactured from transparent, virgin polystyrene grade USP VI in Clean Room conditions; they are free of DNase, RNase, and pyrogens, and the batch number ensures full batch traceability. The design of the plates and inserts is compatible with automatic pipetting systems and standard microscopes and plate readers, and for batch work it is worth choosing automatic pipettes and accessories from the category serological pipettes.